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2024 05 v.54 919-928
川芎嗪抑制ROCK的表达降低模拟失重大鼠血管Ca~(2+)敏感性
基金项目(Foundation): 国家自然科学基金(32071517,32070442); 西北大学大学生创新训练计划项目(2024)
邮箱(Email): gaoyunf@nwu.edu.cn;
DOI: 10.16152/j.cnki.xdxbzr.2024-05-014
中文作者单位:

西北大学生命科学学院/西部资源生物与现代生物技术教育部重点实验室;西北工业大学生命学院/西安市特种医学与健康工程重点实验室;

摘要(Abstract):

研究失重条件下血管平滑肌收缩性、Ca~(2+)敏感性及其调控通路RhoA-ROCK蛋白表达的变化,以及川芎嗪干预对其的影响。大鼠尾部悬吊模拟失重,在机体前、后部分别选取颈总动脉和肠系膜上动脉。在模拟失重大鼠颈总动脉中,由苯肾上腺素(PHE)或KCl诱发的血管收缩性和Ca~(2+)敏感性增强,RhoA激酶2(ROCK II)的表达、肌球蛋白磷酸酶靶亚基1(MYPT1)和肌球蛋白调节轻链(MLC)的磷酸化水平均上升,血管孵育Y-27632(ROCK特异性抑制剂)后可降低以上变化。模拟失重大鼠灌饲川芎嗪亦可降低以上变化。模拟失重后,大鼠肠系膜上动脉的收缩性和Ca~(2+)敏感性、ROCK II的表达、MYPT1和MLC的磷酸化水平降低,血管孵育Y-27632对以上变化无明显作用。模拟失重大鼠灌饲川芎嗪亦对以上变化无明显作用。结果表明,由RhoA-ROCK调控的血管平滑肌Ca~(2+)敏感性的变化可能是失重条件下机体前后部血管收缩性发生区域性重塑的关键因素。川芎嗪可抑制ROCK蛋白的表达,降低血管平滑肌升高的Ca~(2+)敏感性,从而纠正失重条件下机体前部血管收缩性的增强,但对失重条件下机体后部血管收缩性的减弱无恢复作用。

关键词(KeyWords): Ca~(2+)敏感性;RhoA-ROCK;血管收缩性;模拟失重;川芎嗪
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基本信息:

DOI:10.16152/j.cnki.xdxbzr.2024-05-014

中图分类号:R285.5

引用信息:

[1]王慧平,白晓镯,赵晶等.川芎嗪抑制ROCK的表达降低模拟失重大鼠血管Ca~(2+)敏感性[J].西北大学学报(自然科学版),2024,54(05):919-928.DOI:10.16152/j.cnki.xdxbzr.2024-05-014.

基金信息:

国家自然科学基金(32071517,32070442); 西北大学大学生创新训练计划项目(2024)

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