西北大学生命科学学院;陕西省人民医院陕西省感染与免疫疾病重点实验室;
世界范围内,病原菌对抗生素的耐药性日趋严峻,但药物研发效率日趋缓慢,迫切需要新的抗感染药物或药物作用靶点。铜绿假单胞菌群体感应系统(quorum sensing, QS)与其致病性密切相关,寻找靶向QS系统的抑制剂是新型抗感染药物新策略。前期研究发现,以咖啡酸为主要成分的大血藤提取物对铜绿假单胞菌的群体感应具有转录抑制作用。进一步探究了咖啡酸对铜绿假单胞菌的QS系统相关基因的表达和毒性因子产生的抑制作用;通过分子对接结合缺失突变体感染白菜模型检测,探究了咖啡酸的作用靶点。结果表明:咖啡酸显著抑制铜绿假单胞菌QS系统相关基因rhlR、rhlI、pqsA、pqsR及其调控的毒性因子的编码基因如phzA1、lasB等,进一步抑制该类毒性因子的产生和降低了细菌的运动性和生物被膜,最终降低了铜绿假单胞菌的感染性,其可能通过靶向pqs系统发挥其QS抑制作用。
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基本信息:
DOI:10.16152/j.cnki.xdxbzr.2024-05-009
中图分类号:R378
引用信息:
[1]宋文旭,冯玉琦,韩啸枫等.咖啡酸对铜绿假单胞菌群体感应抑制探究[J].西北大学学报(自然科学版),2024,54(05):867-875.DOI:10.16152/j.cnki.xdxbzr.2024-05-009.
基金信息:
陕西省自然科学基金(2018ZDXM-SF-004); 陕西化生基础科学研究项目(22JHZ008); 陕西省感染与免疫疾病重点实验室开放基金(2023-KFZD-3)