| 128 | 2 | 1 |
| 下载次数 | 被引频次 | 阅读次数 |
目的研究频率为2.2MHz的聚焦超声结合血卟啉对在体S180肿瘤组织的杀伤作用。方法计算不同处理后对肿瘤组织的生长抑制作用,并通过透射电镜观察处理后不同时间段取材S180实体瘤超微结构的变化。结果单纯血卟啉对在体S180肿瘤细胞几乎没有生长抑制作用;单纯超声对S180肿瘤组织有一定的生长抑制作用,且随着超声强度的增加,抑制效应逐渐显著,而超声结合血卟啉所产生的协同效应对S180肿瘤细胞的生长抑制作用明显高于单纯超声组,电镜下观察到超声激活血卟啉可以破坏肿瘤细胞的超微结构,尤其是对肿瘤细胞膜和膜性细胞器的通透性和结构的损伤,并且随着取材时间的延长,损伤效应进一步加强。结论超声结合血卟啉能有效地抑制S180肿瘤的生长作用。
Abstract：Aim To study the cell killing effect of sonodynamically activating hematoporphyrin(Hp) on S180 solid tumors with ultrasound at the frequency of 2.2 MHz.Methods The anti-tumor effect was estimated by measuring tumor size and the morphological changes of S180 cells were evaluated by transmission electron microscope(TEM) observation.Results Hp alone had almost no inhibitory effect;ultrasound alone showed some anti-tumor effect,and which became more and more remarkable as ultrasound intensity increased,while the synergistic inhibitory effect of PPIX and ultrasound was more significant than ultrasound alone treatment.TEM observation showed cell morphological changes with severely broken cytoplasm membranes and other membranous organelles might play important roles in resulting in cell damages with SDT,and this damage increased as time delayed after treatment.Conclusion The anti-tumor effect of S180 solid tumors could be significantly induced by focused ultrasound activating hematoporphyrin.
[1]UMEMURA S,YUMITA N,NISHIFAKI R,et al.Sono-chemical activation of hematoporphyrin:A potential mo-dality for cancer treatment[J].IEEE Ultrasonics Symosi-um,1989,9:955-960.
[2]UMEMURA S,YUMITA N,NISHIFAKI R,et al.Mecha-nism of cell damage by ultrasound in combination with he-matoporphyrin[J].Jpn J Cancer Res,1990,81(9):962-966.
[3]QUAN HONG LIU,XIAO BING WANG,PAN WANG,et al.Sonodynamic effects of protoporphyrin IX disodiumsalt on isolated sarcoma 180 cells[J].Ultrasonics,2006,45(1-4):56-60.
[4]李萌,刘全宏,齐浩,等.超声激活血卟啉杀伤癌细胞的扫描电镜观察[J].西北大学学报:自然科学版,2003,33(2):223-225.
[5]WORTHINGTON AE,THOMPSON J,Rauth AM,et al.Mechanism of ultrasound enhanced porphyrin cytotoxicity.Part I:A search for free radical effect[J].Ultrasound inMedicine&Biology,1997,23(7):1 095-1 097.
[6]刘全宏,张坤,张风月,等.超声激活血卟啉对SW-480癌细胞的杀伤作用[J].西北大学学报:自然科学版,2003,33(1):94-98.
[7]YUMITAN,KAWABATAK,SASAKI K,et al.Sonody-namic effect of erythrosin B on sarcoma 180 cells in vitro[J].Ultrasonics Sonochemistry,2002,9(5):259-265.
[8]LIU Quan-hong,SUN Shi-hui,XIAO Ya-ping,et al.Study of cell killing on S180 by different intensity ultra-sound activate hematoporphyrin derivatives[J].Science inChina(Series C),2002,32(5):454-462.
[9]刘全宏,王仲会,任耀辉,等.超声激活血卟啉杀伤S180肉瘤细胞研究初报[J].科学通报,1994,39(10):936-939.
[10]LIU Quan-hong,SUN Shi-hui,XIAO Ya-ping,et al.Synergistic anti-tumor effect of ultrasound and hematopor-phyrin on sarcoma180 cells with special reference to thechanges of morphology and cytochrome oxidase activity oftumor cells[J].J Exp Clin Cancer Res,2004,23(2):333-341.
[11]MUNSON B R.Photodynamic inactivation of mammalianDNA-dependent RNApolymerase by hematoporphyrin andvisible light[J].Int J Biochem,1979,10(12):957-60.
[12]ALLISONAC,MAGNUS I A,YOUNG MR.Role of ly-sosomes and of cell membranes in photosensitization[J].Nature,1966,209(26):874-878.
[13]卢志达,卞明,张新建,等.血卟啉衍生物(HpD)-激光对S180瘤细胞杀伤作用的电镜观察[J].中华医学杂志,1983,63(6):336-338.
基本信息:
DOI：
中图分类号:R73-36
引用信息:
[1]王攀,王筱冰,张丽娜等.聚焦超声激活血卟啉对在体S180肿瘤细胞的抑制作用研究[J].西北大学学报(自然科学版),2009,39(06):1002-1005.
基金信息:
国家自然科学基金资助项目(30270383);; 陕西师范大学大学生开放性实验基金资助项目