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2024 05 v.54 909-918
铜绿假单胞菌prtN突变引起甲氧苄啶耐药
基金项目(Foundation): 陕西省自然科学基金(2019JM-372)
邮箱(Email): chenlin@nwu.edu.cn;
DOI: 10.16152/j.cnki.xdxbzr.2024-05-013
中文作者单位:

西北大学生命科学学院/西部资源生物与现代生物技术教育部重点实验室;

摘要(Abstract):

耐药铜绿假单胞菌(PAO1)在临床感染过程中造成非常棘手的问题,研究其耐药机制有助于临床的治疗。研究结果表明,相对于野生型,ΔprtN表现出明显的甲氧苄啶(Tmp)抗性。为了了解其抗性出现的机理,测定了Tmp作用靶点folA的表达情况。相比于PAO1,folA在ΔprtN中的表达并未升高,反而有所下降。进一步研究发现,PrtN调控的S型绿脓杆菌素基因和prtN的双突变体对Tmp的抗性稍有降低,而脂多糖缺陷菌株ΔwbpL对Tmp的抗性略有升高。在ΔprtN中活性氧(ROS)相关基因(oxyR,katA,ahpC)的表达水平、生物被膜及外排泵相关抗生素抗性检测结果都显示与野生型无显著性差异。综上,prtN基因突变引起的Tmp抗性可能是通过PrtN调控S型绿脓杆菌素及脂多糖相关靶标作用的结果。

关键词(KeyWords): 绿脓杆菌素;prtN;甲氧苄啶;脂多糖;ROS
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基本信息:

DOI:10.16152/j.cnki.xdxbzr.2024-05-013

中图分类号:R378

引用信息:

[1]肖悦,王冲,司玉洁等.铜绿假单胞菌prtN突变引起甲氧苄啶耐药[J].西北大学学报(自然科学版),2024,54(05):909-918.DOI:10.16152/j.cnki.xdxbzr.2024-05-013.

基金信息:

陕西省自然科学基金(2019JM-372)

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