| 182 | 27 | 3 |
| 下载次数 | 被引频次 | 阅读次数 |
采用1.1MHz,0.9W/cm2低强度超声,结合血卟啉对体外培养的人肿瘤细胞SW-480进行声照处理,通过噻唑蓝(MTT)法、PI,HO双荧光染色等方法,根据癌细胞形态结构和功能的变化,探讨超声激活血卟啉对肿瘤细胞的杀伤作用。结果表明:血卟啉对癌细胞无明显的损伤,超声加血卟啉处理30s是引起SW-480细胞受损的敏感位点,90s为杀伤癌细胞的优选时间段;杀伤作用呈癌细胞显微结构逐步破坏的变化,并随声照时间的延长而加剧;声照时间在30s至90s区间细胞具有明显凋亡特征,提示超声激活血卟啉杀伤癌细胞机制中可能存在诱导肿瘤细胞凋亡的作用。
Abstract：The combined use of ultrasound and HpD were studied at lower frequency of 1.1 MHz and intensity of 0.9W/cm2.MTT method,morphological observation and doublefluos staining method were used to study the combined killing faculty of ultrasound and HpD to tumor cells according to morphological and functional variation.HpD has little effort on tumor cells, 30s is the sensitive threshold value when insonated by ultrasound combined use HpD and 90s is the best period to kill the tumor cells.The damage became aggravating with time passing.SW480 cells have apoptosis character during 30 s and 90 s,which indicates that the mechanism of killing cells has the apoptosis mode when induce the cancer cells to death.
[1] YUMITAN,NISHIGAKIR,UMEMURAK,etal.Hematoporphyrinasasensitiverofcell-damagingef-fectofultrasound[J].JpnJCancerRes,1989,80(3):219-222.
[2] YUMITAN,NISHIGAKIR,UMEMURAK,etal.Synergisticeffectofultrasoundandhematoporphyrinonsarcoma180[J].JpnJCancerRes,1990,81:304-308.
[3] AMSONN.Applicationsoftherapeuticultrasoundinmedicine[J].UltrasonicsSonochemistry,1994,(1):69-71.
[4] UMEMURAS,KAWABATAK,SASAKIK,etal.Recentadvancesinsonodynamicapproachtocancertherapy[J].UltrasonicsSonochemistry,1996,(3):187-191.
[5] MIYOSHIN,MISKIV,FUKUDAM,etal.EffectofgalliumporphyrinanalogueATX-70onnitroxidefor-mationfromacyclicsecondaryaminabyultrasound:onthemechanismofsonodynamicactivition[J].RadiatRes,1995,143(2):194-202.
[6] SAKUSABEN,OKADAK,SATOK,etal.Enhancedsonodynamicantitumoreffectofultrasoundinthepresenceofnonsteroidalanti-inflammatorydrugs[J].JpnJCancerRes,1999,90(10):1146-1151.
[7] MISIKV,RIESZP.Freeradicalintermediateinsono-dynamictherapy[J].AnnNYAcadSci,2000,899:335-348.
[8] YUMITAN,UMEMURAS,NISHIGAKIR.Ultra-sonicallyinducedcelldamageenhancedbyphotofrin:mechanismofsonodynamicactivation[J].InVivo,2000,14(3):425-429.
[9] MIYOSHIN,IGARASHIT,RIESZP.Evidencea-gainstsingletoxygenformationbysonolysisofaque-ousoxygen-saturatedsolutionsofHematoporphyrinandrosebengal.themechanismofsonodynamicther-apy[J].UltrasonSonochem,2000,7(3):121-124.
[10] KESSELD,JEFFERSR,FOWLKESJB,etal.Ef-fectsofsonodynamicandphotodynamictreatmentoncellularthiollevels[J].PhotochemPhotobiolB,1996,32(1-2):103-106.
[11] MISIKV,RIESZP.PeroxylradicalformationinaqueoussolutionsofN,N-dimethylformamide,N-methylformamide,anddimethylsulforxidebyultra-sound:implicationsforsonosensitizedcellkilling[J].FreeRadicRes,1996,25(1):13-22.
[12] UCHIDAT,TACHIBANAK,HISANOS,etal.E-liminationofadultTcellleukemiacellsbyultrasoundinthepresenceofporfimersodium[J].AnticancerDrugs,1997,8(4):329-335.
[13] MIYOSHIN,MISIKV,RIESZP.Sonodynamictoxi-cityofgallium-porphyrinanalogueATX-70inhumanleukemiacell[J].RadiatRes,1997,148(1):43-47.
[14] WORTHINGTONAE,THOMPSONJ,RAUTHAM,etal.(Mechanismofultrasoundenhancedpor-phyrincytotoxicity:Asearchforfreeradicaleffect)[J].UltrasoundMedBiol,1997,23(7):1095-1105.
[15] HRISTOVPK,PETROVLA,RUSSANOVEM.LipidperoxidationinducedbyultrasonicationinEhrlichascitictumorcells[J].CancerLett,1997,121(1):7-10.
[16] 齐 浩,谭声江,马玉英,等.不同频率聚焦超声与血卟啉对人体肿瘤细胞的协同作用[J].中国科学(C辑),1998,28(5):477-480.
[17] 刘全宏,王仲会,任耀辉,等.超声激活血卟啉杀伤S180肉瘤细胞研究初报[J].科学通报,1994,39:936-938.
[18] ASHUSHH,ROZENSZAJNLA,BLASSMetal.Apoptosisinductionofhumanmyeloidleukemiccellsbyultrasoundexposure[J].CancerRes,2000,60(4):1014-1020
基本信息:
DOI：
中图分类号:R730.59
引用信息:
[1]刘全宏,张坤,张凤月,齐浩,尚志远,姚潇.超声激活血卟啉对SW-480癌细胞的杀伤作用[J].西北大学学报(自然科学版),2003(01):94-98.
基金信息:
国家自然科学基金资助项目(39870240);; 教育部"高等学校骨干教师资助项目