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目的从分子水平证实空间搭载对梭梭种子后代的遗传物质发生变异。方法利用"神舟"四号飞船搭载的梭梭种子地面种植,进行简单重复间序列(inter-simple sequence repeats,ISSR)检测。结果空间搭载的梭梭和地面对照经ISSR分析,94个ISSR引物中,80个引物扩增的DNA带型一致,14个引物扩增的DNA带型表现多态性。空间搭载的梭梭和地面对照相比,共发现5个特异位点,分别为UBC834-1000,UBC834-1200,UBC840-3000,UBC841-2000和UBC846-1100。另外,地面对照组的多态位点比率(P)、Nei基因多样度(h)和Shannon多样性指数(I)均低于空间搭载组。结论空间环境可引起梭梭遗传物质DNA水平上的变异。
Abstract：Aim To identify the variation of hereditary substance of H.ammodendron offspring from seeds under "Shenzhou No.4" spacecraft condition.Methods Haloxylon ammodendron seeds carried in "Shenzhou No.4" spaceflight within ground-based control were planted on the ground.The young fresh shoots of plants were used to do inter-simple sequence repeats analysis.Results Ninety-four ISSR primers were used in this study,among which 80 primers generated the same DNA band type,and 14 primers generated different DNA band types.Compared with the control,5 ISSR specific markers were obtained,namely UBC834-1000,UBC834-1200,UBC840-3000,UBC841-2000 and UBC846-1100.Moreover,the percentage of polymorphic loci(P),Nei′s gene diversity(h) and Shannon′s index(I) in H.ammodendron seeds of the control was lower than that of the seeds carried in "Shenzhou No.4" spaceflight.Conclusion Space environment can cause variation of hereditary substance DNA of H.ammodendron.
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基本信息:
DOI：
中图分类号:S793.9
引用信息:
[1]王戌梅,杨冬野,田永祯,等.“神舟”四号飞船搭载的梭梭ISSR分析[J].西北大学学报(自然科学版),2009,39(02):259-263.
基金信息:
国家重大基础研究专项基金资助项目(2004CCA01200)